Comparative analysis of the characteristics of Haplorchis taichui mitochondrial genome with Metagonimus yokogawai and its ribosomal transcription unit with H. pumilio (family Heterophyidae)

Minute intestinal flukes, Haplorchis taichui and H. pumilio, belong to the family Heterophyidae (Trematoda: Platyhelminthes), which have been studied very limited, especially molecular markers of mitochondrial genomes (mtDNA) ribosome transcription units (rTU or rDNA). We obtained complete genome coding part unit pumilio Vietnam. Nucleotide amino acid data were compared between Metagonimus yokogawai for genomic/gene composition, codon usage (skew/skewness), tandem repeat (TRU). The mtDNA (strain Htai-QT3-VN) with length 15,120 bp M. (15,258 bp; Korea; KC330755) contains 36 genes, including 12 protein-coding genes (cox1, cox2, cox3, nad1, nad2, nad3, nad4L, nad4, nad5, nad6, atp6 cob), 2 ribosomal RNA (rRNA); 22 transfer (tRNA trn) a noncoding region (NCR) trnE trnG, divided into sub-regions containing 5 TRUs (182–183 bp/TRU). (Vietnam Laos) uses A = 19.56%, T 39.71%, G 28.34%, C 12.39% (A + is 59.27% ​​and 40.73%) construction, whose skew/skewness value at A+T negative (–0,340) G+C positive (0.392); (PCGs) similar; but mito-ribosomal (MRGs, 16S/rrnL 12S/rrnS) it less (57.22%) more (42.78%). had lower (mtDNA/55.68%; PCGs/55.96%; MRGs/54.15%) higher rate than taichui. Vietnam Laos has 10,164 encoding 3,376 acids construct PCGs mostly used codons as Phenylalanine (Phe-TTT) Leucine (Leu-TTG), leastly codonsas Glutamine (Gln-CAA), Arginine (Arg-CGC). Additional condon, Thr-ACA/ACC can be added least in yokogawai. rTU (from '18S 3' 28S) (7,268 bp) (7,416 identified genomic regions 18S rDNA, ITS1, 5.8S ITS2 28S rDNA. both species same (1,992 18S, 160 5.8S), different (3,875 3,870 pumilio). ITS1 (797 (280 do not contain TRUs, whilst (1,106 TRUs(136 3 TRU 116 TRUs); (444 (83–85 bp/each).